Fourier transform infrared spectroscopy analysis of Lactose hydrolysis by beta-galactosidase from Lactiplantibacillus plantarum GV54 and Lactiplantibacillus sp. GV66

Introduction and Aim: The enzyme beta-galactosidase is extremely important in the food pharmaceutical industries. goal of this work was to improve lactose hydrolysis process employing convert glucose galactose for alleviating intolerance. Methods: Intracellular extracted from Lactiplantibacillus spp. (GV54 GV66) by cell permeabilized method using toluene/acetone solvents. Lactose performed 50 g/L with partially purified at different time intervals 2, 4, 6 12 h. Fourier transforms infrared spectroscopy (FTIR) used evaluate degree high h incubation. Results: FTIR peak molecules (2h, 4h, 6h, 12h) indicates similarities bond lengths vibrational modes. amide I group uniformly bound 1634 per cm C-H observed 1386 cm. In range 2000-800 cm, we could identify variations shape strength these peaks, compared control (lactose solution). Conclusion: precise direct measurement individual carbohydrates such as solution hydrolyzed successfully accomplished spectrometry. Comparing analysis traditional procedures, required drastically decreased. These findings have analytical power follow dynamics vitro enzymatic activity naturally occurring substrates rich natural carbohydrate polymers.